Wheat is a global staple food and plays a pivotal role in the livelihoods of billions of people. Although long non-coding RNAs (lncRNAs) have been recognized as crucial regulators of numerous biological processes, our knowledge of lncRNAs associated with wheat (Triticum aestivum) grain development remains minimal. Seed Biology published an open-access online paper entitled “A Comprehensive Atlas of Long Non-Coding RNAs Provides Insight Into Grain Development In Wheat” on September 4, 2023 . To elucidate the landscape of lncRNAs in wheat, this study conducted genome-wide strand-specific RNA sequencing (ssRNA-seq) on grain endosperm at 10 and 15 days after pollination (DAP) and integrated 545 publicly available transcriptome datasets from various developmental stages and tissues. The analysis pipeline was adapted from a previous study with modifications, processed RNA-seq data in three primary steps: mapping, assembly, and filtering. The results identified 20,893 lncRNAs in wheat.