Virus “Barcodes” Offer Rapid Detection of Mutated Strains

Dr. Julian Hiscox and Dr. John Barr of the University of Leeds’ Faculty of Biological Sciences are working with the Health Protection Agency (HPA) Porton to build a bank of molecular signatures that will help identify the severity of virus infection from characteristic changes seen in cells. Currently the team is barcoding different strains of influenza virus and human respiratory syncytial virus (HRSV) - a virus associated with the onset of asthma in young children. "Diseases such as flu infect and hijack our cells, turning them into virus-producing factories," says Dr. Hiscox. "The infection causes the balance of proteins in a cell to change - some proteins are overproduced and others suppressed. Which proteins are affected and by how much varies depending on the type of virus, allowing us to identify a unique barcode of disease for each." The research, published May 14, 2012 in Proteomics, investigates changes in lung cells infected with swine flu from the 2009 outbreak compared with seasonal flu. The team used a labeling technique called SILAC to measure and compare thousands of different proteins in a sample. This technique was used alongside mass spectrometry to identify the proteins most affected by viral infection and used these as molecular signatures to provide the “barcode” of disease. The paper reports how several processes in the cell were affected by the virus, with most changes seen in proteins involved in cell replication. "Swine flu affects the lungs in a similar way to seasonal flu and this was reflected in the barcodes we found for each," explains Dr. Barr. "Using this test might have been a way to identify how lethal the 2009 swine flu pandemic was going to be, lessening worldwide panic.
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