Single-Molecule DNA Sequencing Enables ID of Putative Coffee Rust Mycoparasites; Coffee Rust Fungus Is Ravaging Latin America Coffee Plantations; Mycoparasites of Coffee Rust May Aid Biocontrol

Coffee rust has ravaged Latin American plantations for several years, leading to reductions in annual coffee production of up to 30 percent in some countries and threatening the livelihoods of hundreds of thousands of small-scale farmers in the region. A new study by University of Michigan (U-M) researchers suggests that the coffee plants themselves may hold biological weapons that might someday be harnessed in the fight against the coffee rust fungal pathogen. Those potential weapons are themselves also fungi, a surprisingly diverse community of more than 300 species of them--including 15 likely fungal parasites--living on coffee leaves, within or alongside the yellow blotches (image) that mark coffee rust lesions. Using an old-fashioned handheld paper punch, U-M researchers collected leaf samples from both infected and uninfected coffee leaves at coffee farms in Chiapas, Mexico, and in Puerto Rico. They found up to 69 fungal species living on a single quarter-inch-diameter leaf disc from uninfected leaves and up to 62 species on rust-infected leaf discs, according to Timothy James, Ph.D., a U-M mycologist and lead author of a paper published online on November 13, 2015 in Applied and Environmental Microbiology, a journal of the American Society for Microbiology. The paper is titled “Identification of Putative Coffee Rust Mycoparasites Using Single Molecule DNA Sequencing of Infected Pustules." "Latin America is experiencing unprecedented epidemics of coffee rust, so identification of its natural enemies could aid in developing management strategies or in pinpointing species that could be used for biocontrol," said Dr. James, an Associate Professor in the Department of Ecology and Evolutionary Biology (EEB) at U-M.
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