Rutgers Scientists Combine Several Capabilities for First Time in Device Called “ELISA-on-a-Chip;” Predicted to Slice Cost of Lab Tests for Wide Range of Disorders, Including HIV, Lyme Disease, and Syphilis; Requires 90% Less Fluid Than Current Tests

Rutgers engineers have developed a breakthrough device that can significantly reduce the cost of sophisticated lab tests for medical disorders and diseases, such as HIV, Lyme disease, and syphilis. The new device uses miniaturized channels and valves to replace "benchtop" assays - tests that require large samples of blood or other fluids and expensive chemicals that lab technicians manually mix in trays of tubes or plastic plates with cup-like depressions. "The main advantage is cost - these assays are done in labs and clinics everywhere," said Dr. Mehdi Ghodbane, who earned his doctorate in biomedical engineering at Rutgers and now works in biopharmaceutical research and development at GlaxoSmithKline. Dr. Ghodbane and six Rutgers researchers first published their results online on June 23, 2015 in the Royal Society of Chemistry's journal, Lab on a Chip. The article is titled “Development and Validation of a Microfluidic Immunoassay Capable of Multiplexing Parallel Samples in Miicroliter Volumes.” The lab-on-chip device, which employs microfluidics technology, along with making tests more affordable for patients and researchers, opens doors for new research because of its capability to perform complex analyses using 90 percent less sample fluid than needed in conventional tests. "A great deal of research has been hindered because in many cases one is not able to extract enough fluid," Ghodbane said. The Rutgers breakthrough also requires one-tenth of the chemicals used in a conventional multiplex immunoassay, which can cost as much as $1500. Additionally, the device automates much of the skilled labor involved in performing tests. "The results are as sensitive and accurate as the standard benchtop assay," said Dr.
Login Or Register To Read Full Story