With a near-record attendance of 1,175 scientists from 44 countries and a record number of sponsors (37), the recently concluded International Society for Extracellular Vesicles (ISEV) annual meeting 2023 (May 17-21) in Seattle, Washington, signaled the surge of progress in the study of extracellular vesicles (EVs), including exosomes, in different organisms (bacteria, plants, pathogenic parasites, and animals) and for all manner of purposes. Key talks on Sunday’s closing day focused on the advance of preclinical research toward clinical trials and the study of EV changes in the four civilian astronauts who had orbited the earth in the Space-X first all-civilian spaceflight. Earlier presentations included a poster on identification of EVs in mares in Kazakhstan and one on exosomes in cow’s milk. Sunday also featured a closing ceremony that included announcement of awards for the best oral and best poster presentations. In all, there were numerous oral presentations and panel discussions, 50 posters, 4 plenary sessions, and 3 featured abstracts in the four days of the meeting. Also, a full Education Day preceded the main meeting.