Novel Exosome Isolation and Exosomal MicroRNA Purification Kits Launched by Canada’s Norgen Biotek

Norgen Biotek, Corp., an innovative privately-held Canadian biotechnology company that is focused on advancing powerful tools for nucleic acid (NA) and protein purification, announced on Monday, November 2, 2015, the launch of novel kits for the fast and simple isolation of exosomes from plasma/serum, urine, and cell culture media. These kits are based on the use of Norgen's proprietary resin to purify and concentrate the exosomes. The company says these new kits are simple, rapid, and scalable. Furthermore, Norgen said it has developed kits for the isolation of high-quality RNA from exosomes. Norgen's exosomal RNA kits are designed to isolate all sizes of extracellular vesicle (EV) RNA, including microRNA. The company believes that the kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, or phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into flexible elution volumes. Norgen states that the purified RNA is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real-time PCR, RT-PCR, Northern blotting, RNase protection, primer extension, expression array assays, and next-generation sequencing, the company states. Norgen also launched four kits that allow for the depletion of exosomes from fetal bovine serum. Extensive testing has shown that depleted FBS provides the same cellular growth rates as the standard FBS. "The study of exosomes is an exciting new field, and Norgen's technology is well suited for the purification of exosomes and exosomal RNA,” said Dr. Yousef Haj-Ahmad, President & CEO of Norgen.
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