Next-Gen DNA Sequencing Reveals Secrets of White Cliffs of Dover

The University of Exeter recently contributed to a major international project to sequence the genome of Emiliania huxleyi, the microscopic plankton species whose chalky skeletons form the iconic white cliffs of Dover. The results of the project were published online on June 12, 2013 in an open-access article in Nature. Emiliania huxleyi is one of the most abundant marine phytoplankton species and is a key player in the process of CO2 exchange between the atmosphere and the ocean. In some marine systems 20% of the total carbon is fixed by E. huxleyi. This microscopic alga has influenced the global climate for over 200 million years, so is used as a model system for studying how physical, chemical, and biological processes regulate the Earth's systems. The algae form pale chalky cases called coccoliths which during the spring bloom can be seen from space in the seas around the UK. E. huxleyi directly links to climate change through the production of dimethylsulfide (DMS), which induces cloud formation and blocks solar radiation. Thanks to new technology – next-generation DNA sequencing – 13 different isolates were sequenced from around the world, and compared to a complete sequence constructed for E. huxleyi strain CCMP1516. This allowed the team to understand the influences of different environmental conditions on E. huxleyi physiology. The international team found that E. huxleyi possess a higher number of genes than previously published marine phytoplankton genomes, and that most genes were present in multiple copies. Dr Mark Van Der Giezen from the University of Exeter said: "Using comprehensive analysis to compare different strains of the algae, we demonstrated that E. huxleyi should no longer be considered a single species.
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