Bacterial Origins of Trench Fever in Humans Traced Back Centuries by Real-Time PCR-Based DNA Analysis of Ancient Remains; Remnants of Infections Demonstrated in Civilians As Well As Soldiers

First observed among British Expeditionary Forces in 1915, trench fever sickened an estimated 500,000 soldiers during World War I. Since then, the disease has become synonymous with the battlefield. But now, new research from an international team of scientists has uncovered evidence challenging this long-held belief. The research, published online on November 4, 2020 in PLOS ONE, outlines the discovery of DNA evidence of the disease in civilian remains predating WWI by thousands of years. In total, the team analyzed bone fragments and teeth of 145 individuals alive between the 1st and 19th centuries. Approximately 20% of those remains contained traces of Bartonella quintana, the bacteria responsible for trench fever. Davide Tanasi, PhD, an Associate Professor with the University of South Florida (USF) History Department and member of the Institute for the Advanced Study of Culture and the Environment, excavated remains for this project from a Roman cemetery in Syracuse, Sicily. Dr. Tanasi, who is also the Director of USF's Institute for Digital Exploration, first began work at the site to better understand the dietary habits and health of the Christian population living there during the 3rd and 4th centuries. Through a collaboration with French epidemiologists, researchers used real-time polymerase chain reaction (PCR) testing to detect B. quintana DNA within the remains. "Once contracted, there are diseases, like trench fever, that can leave traces within your DNA and can integrate your DNA with further information," Dr. Tanasi said. "This means that once a person dies, even as far back as 2,000 years ago, it is still possible to find traces of the bacterium that infected them."
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